Our Research

RVU120

CDK8 is a kinase submodule of the mediator complex, involved in both transcriptional activation and repression. CDK8- mediator complex integrates basal transcriptional machinery with the activity of oncogenic transcriptional and epigenetic factors. Targeting CDK8 and its paralog CDK19 using potent and selective CDK8 inhibitor RVU120 may be an effective way for treatment of both hematological malignancies and solid tumors with deregulated transcription.  Functional studies defined CDK8 as a regulator of paracrine activities of cancer cells, hypoxia response network and other pathways including the p53 network, the Wnt–β-catenin pathway, the Notch1,  JAK/STAT1 signaling, and pathways regulated by SMAD TFs. Because of the established role in repression of NK cell cytotoxicity and tumor surveillance, CDK8 inhibitors may also have utility as an immunooncology treatment.

In March 2019 Ryvu received approval from the U.S. Food and Drug Administration to initiate a Phase 1b study for its RVU120 program in acute myeloid leukemia (AML) and high-risk myelodysplastic syndromes (HR-MDS) and first patient was dosed in September 2019. On March 25, 2020, the U.S. Food and Drug Administration (FDA) granted an orphan drug designation (ODD) to RVU120, for the treatment of patients with acute myeloid leukemia (AML).

The first data from the program, comprising the first five evaluable patients, presented at EHA 2021 conference, demonstrated favorable safety profile and initial single agent activity (complete response in AML and erythroid response in MDS).

In addition, RVU120 has demonstrated single agent efficacy in multiple solid tumor models. On August 25, 2021, the first patient enrolled in the Phase I/II clinical trial investigating RVU120 (SEL120) in relapsed/refractory metastatic or advanced solid tumors, has received the first dose of the study drug.

KEY FACTS:

• RVU120 is a selective CDK8/CDK19 inhibitor in Phase I in patients with AML/ HR-MDS (NCT04021368), and Phase I/II in relapsed/refractory metastatic or advanced solid tumors
• RVU120 treatment results in on-target efficacy in preclinical models of AML in vitro and in vivo
• Inhibition of CDK8 can repress key oncogenic transcriptional programs and induce lineage commitment genes in AML
• CDK8 and CDK19 are also preclinically validated novel targets for the treatment of breast and prostate cancers
• There are no competitive CDK8/19 inhibitors actively tested in clinical trials

RYVU’s APPROACH:

• RVU120 is a clinical stage highly specific and orally bioavailable dual CDK8/CDK19 inhibitor
• First patient dosed in Phase Ib trial in AML and HR-MDS in August 2019
• First patient dosed in Phase i/II trial in solid tumors in August 2021
• Demonstrated high on-target efficacy, including complete regressions in preclinical models of AML
• Efficacy in leukemia cells linked with induction of apoptotic cell death and induction of differentiation
• Demonstrated synergy with standard of care and late stage development candidate drugs in AML
• Specific inhibition of CDK8/19 biomarkers correlates with efficacy in responder cells in the low nM range
• On-target transcriptional changes in cancer cells with RVU120, including inhibition of oncogenic gene expression patterns and induction of lineage commitment genes
• Bioinformatic analysis of treated cells allowed for in-depth understanding of a link between molecular mode of action and response in cancer cells
• Differential efficacy of RVU120 demonstrated also in breast cancer, Willms tumors, Lymphomas and colorectal cancer models in vitro and in vivo
• Synergy of RVU120 with anti-PD1 antibodies in immunooncology in vivo models
• RVU120 treatment effectively increases reticulocyte and hemoglobin levels in models of a rare ribosomopathy – Diamond-Blackfan Anemia
• Partnership with The Leukemia & Lymphoma Society to co-fund and scientifically support further preclinical and clinical development of RVU120

• DECEMBER 2022

  Single-agent activity of RVU120 with a complete response, 4 blast reductions, and 4 erythroid and/or platelet responses in patients with r/r AML or HR-MDS, presented at the ASH 2022 conference.

• OCTOBER 2022

  Updated data from Phase I/II dose-escalation study of RVU120 with 4 disease stabilizations in advanced solid tumor patients, released at the ENA 2022 conference.

• JUNE 2022

  Clinically meaningful benefit of RVU120 monotherapy: a complete remission in an AML patient with FLT3/DNMT3A/NPM1 mutations; more than 18-month duration of therapy in a DNMT3A-mutated high-risk MDS patient; stable diseases with blast reductions in 3 additional patients, demonstrated at the EHA 2022 Congress.

• DECEMBER 2021

  Single-agent activity of RVU120 (complete response in r/r AML and erythroid response in HR-MDS), as well as an acceptable safety profile in 6 patients with no dose-limiting toxicity or treatment-related SAEs, presented at the ASH 2022 conference.

• AUGUST 2021

  First patient dosed in Phase I/II study in patients with relapsed/refractory metastatic or advanced solid tumors.

• JUNE 2021

  The first data from the program, comprising the first five evaluable patients, presented at EHA 2021 conference, demonstrated favorable safety profile and initial single agent activity (complete response in AML and erythroid response in MDS).

• MAY 2021

  Ryvu’s Clinical Trial Application (CTA) to commence a single-agent, open-label Phase I/II trial, investigating the safety and efficacy of RVU120 (SEL120) in patients with relapsed/refractory metastatic or advanced solid tumors, was approved by the Polish Office for Registration of Medicinal Products, Medical Devices and Biocidal Products, and the respective Central Ethics Committee.

• MARCH 2020

  Ryvu received Orphan Drug Designation from FDA for RVU120 to treat Acute Myeloid Leukemia.

• SEPTEMBER 2019

  First patient dosed in Phase 1b trial in AML and HR-MDS patients.

• MARCH 2019

  Ryvu received approval from the U.S. Food and Drug Administration to initiate a Phase 1b study for its RVU120 program in acute myeloid leukemia and high-risk myelodysplastic syndromes.

• AUGUST 2017

  Ryvu has initiated a partnership with The Leukemia & Lymphoma Society to co-fund and scientifically support further preclinical and clinical development of RVU120 as a targeted therapy to treat patients with acute myeloid leukemia.

Ryvu poster publications:

RECOMMENDED LITERATURE:

• clinicaltrials.gov (https://clinicaltrials.gov/ct2/show/NCT04021368)

REVIEW ARTICLES

• Philip, Stephen, Malika Kumarasiri, Theodosia Teo, Mingfeng Yu, and Shudong Wang, ‘Cyclin-Dependent Kinase 8: A New Hope in Targeted Cancer Therapy?’, Journal of Medicinal Chemistry, 2017, acs.jmedchem.7b00901 https://doi.org/10.1021/acs.jmedchem.7b00901
• Roninson, Igor B., Balázs Győrffy, Zachary T. Mack, Alexander A. Shtil, Michael S. Shtutman, Mengqian Chen, and others, ‘Identifying Cancers Impacted by CDK8/19’, Cells, 8 (2019), 821 https://doi.org/10.3390/cells8080821
• Rzymski, Tomasz, Michał Mikula, Katarzyna Wiklik, and Krzysztof Brzózka, ‘CDK8 Kinase–An Emerging Target in Targeted Cancer Therapy.’, Biochimica et Biophysica Acta, 1854 (2015), 1617–29 https://doi.org/10.1016/j.bbapap.2015.05.011

SCIENTIFIC PAPERS

• Rzymski, Tomasz, Michał Mikula, Eliza Żyłkiewicz, Agnieszka Dreas, Katarzyna Wiklik, Aniela Gołas, and others, ‘SEL120-34A Is a Novel CDK8 Inhibitor Active in AML Cells with High Levels of Serine Phosphorylation of STAT1 and STAT5 Transactivation Domains’, Oncotarget, 8 (2017), 33779–95 https://doi.org/10.18632/oncotarget.16810
• Nitulescu, Ioana I., Sara C. Meyer, Qiang Jeremy Wen, John D. Crispino, Madeleine E. Lemieux, Ross L. Levine, and others, ‘Mediator Kinase Phosphorylation of STAT1 S727 Promotes Growth of Neoplasms With JAK-STAT Activation’, EBioMedicine, 26 (2017), 112–25 https://doi.org/10.1016/j.ebiom.2017.11.013
• Pelish, Henry E, Brian B Liau, Ioana I Nitulescu, Anupong Tangpeerachaikul, Zachary C Poss, Diogo H Da Silva, and others, ‘Mediator Kinase Inhibition Further Activates Super-Enhancer-Associated Genes in AML.’, Nature, 2015 https://doi.org/10.1038/nature14904
• Xu, Dazhi, Chien-Feng Li, Xian Zhang, Zhaohui Gong, Chia-Hsin Chan, Szu-Wei Lee, and others, ‘Skp2-MacroH2A1-CDK8 Axis Orchestrates G2/M Transition and Tumorigenesis.’, Nature Communications, 6 (2015), 6641 https://doi.org/10.1038/ncomms7641
• McDermott, Martina S.J., Alexander A. Chumanevich, Chang Uk Lim, Jiaxin Liang, Mengqian Chen, Serena Altilia, and others, ‘Inhibition of CDK8 Mediator Kinase Suppresses Estrogen Dependent Transcription and the Growth of Estrogen Receptor Positive Breast Cancer’, Oncotarget, 8 (2017), 12558–75 https://doi.org/10.18632/oncotarget.14894
• Hsieh, Robert W, Angera H Kuo, Ferenc A Scheeren, Mark A Zarnegar, Shaheen S Sikandar, Jane Antony, and others, ‘CDK19 Is a Regulator of Triple-Negative Breast Cancer Growth’, BioRxiv, 2018 https://doi.org/https://doi.org/10.1101/317776
• Bra gelmann, J., N. Klu mper, Anne Offermann, A. von Ma ssenhausen, D. Bo hm, M. Deng, and others, ‘Pan-Cancer Analysis of the Mediator Complex Transcriptome Identifies CDK19 and CDK8 as Therapeutic Targets in Advanced Prostate Cancer’, Clinical Cancer Research, 2016 https://doi.org/10.1158/1078-0432.CCR-16-0094
• Witalisz-Siepracka, Agnieszka, Dagmar Gotthardt, Michaela Prchal-Murphy, Zrinka Didara, Ingeborg Menzl, Daniela Prinz, and others, ‘NK Cell-Specific CDK8 Deletion Enhances Antitumor Responses’, Cancer Immunology Research, 2018, canimm.0183.2017 https://doi.org/10.1158/2326-6066.CIR-17-0183

SEL24 (MEN1703)

SEL24 (MEN1703) is a selective, dual inhibitor of PIM and FLT3 kinases, two enzymes that are strongly implicated in malignant transformation of hematopoietic cells. The compound is a novel small molecule discovered by Ryvu and currently in development in a Phase I/II clinical trial in acute myeloid leukemia (AML ). In March 2017 Ryvu entered into exclusive license agreement with Menarini Group. Menarini has global rights for further development of the compound and is a sole sponsor of the study.

In March 2020, Phase I dose escalation study was successfully completed with the establishment of the recommended dose for Phase II and evidence of single agent efficacy in heavily pretreated AML patients. In addition data presented at EHA 2021 conference, further confirmed single agent activity of the molecule, especially in R/R AML patients harboring IDH mutation.

On November 4, 2021, the U.S. Food and Drug Administration (FDA) granted an orphan drug designation (ODD) to SEL24 (MEN1703) for the treatment of patients with acute myeloid leukemia (AML).

In December 2021, Ryvu licensee Menarini Group presented updated Phase 2 data for SEL24 (MEN1703) (NCT03008187), showing pharmacodynamics (PD) and genomic profiling in the First-in-Human Diamond-01 trial.

Moreover, additional clinical updates for SEL24 (MEN1703) were reported at the EHA 2022 Congress. SEL24 (MEN1703) was well tolerated, with no drug discontinuations or deaths due to treatment-related adverse events (TRAEs). Promising efficacy was observed, with overall response rates (ORR) and complete remission (CR) / CR with incomplete hematologic recovery (CRi) / CR with partial hematologic recovery (CRh) of 13% for the IDHm cohort, which is similar to monotherapy activity of other drugs in R/R AML.

KEY FACTS:

• SEL24 is a selective, orally bioavailable dual PIM/FLT3 inhibitor with successfully completed Phase I trial in patients with AML (NCT03008187)
• PIM and FLT3 are oncogenes involved in AML
• Dual targeting creates potential for broader activity and more durable responses than selective FLT3 inhibitors
• Potential for treating patients that have relapsed on selective FLT3 inhibitors – PIM kinases are largely responsible for the development of resistance to FLT3 inhibitors
• PIM kinases are also involved in other types of different malignancies, including lymphomas and solid tumors
• Project was developed by Ryvu up to initiation of phase I clinical studies in 2017, with first patient dosed in the study in March 2017
• Phase I dose escalation study was successfully completed with the establishment of the recommended dose for Phase II studies of the drug in March 2020
• Partnered globally with Menarini in 2017
• Menarini is currently fully responsible for clinical development
• Menarini plans to continue the study in Phase II (cohort expansion) at the recommended dose level

• JUNE 2022

  Promising efficacy of SEL24 (MEN1703) from an additional expansion cohort in the IDHm AML patients, reported at the EHA 2022 Congress.

• DECEMBER 2021

  Updated Phase 2 data for SEL24 (MEN1703), showing pharmacodynamics (PD) and genomic profiling in the First-in-Human Diamond-01 trial, demonstrated at the ASH 2022 conference.

• NOVEMBER 2021

  Orphan Drug Designation from FDA for SEL24 (MEN1703) to treat Acute Myeloid Leukemia.

• JUNE 2022

  Promising efficacy of SEL24 (MEN1703) from an additional expansion cohort in the IDHm AML patients, reported at the EHA 2021 Congress.

• SEPTEMBER 2020

  First patient dosed in Europe within the Expansion Cohort of Phase I/II Clinical Study of SEL24/MEN1703 in Acute Myeloid Leukemia.

• MARCH 2020

  Successful Completion of Phase I Clinical Study of SEL24/MEN1703 in Acute Myeloid Leukemia.

• MARCH 2017

  Selvita and Menarini Group announce global license agreement for SEL24.

Ryvu poster publications:

RECOMMENDED LITERATURE:

• clinicaltrials.gov (https://clinicaltrials.gov/ct2/show/NCT03008187)

REVIEW ARTICLES:

• PIM kinase inhibition: co-targeted therapeutic approaches in prostate cancer Published: 31 January 2020, Sabina Luszczak, Christopher Kumar, Vignesh Krishna Sathyadevan, Benjamin S. Simpson, Kathy A. Gately, Hayley C. Whitaker & Susan Heavey
  Signal Transduction and Targeted Therapy volume 5, Article number: 7 (2020)
  https://www.nature.com/articles/s41392-020-0109-y

• J Cancer Prev. 2018 Sep; 23(3): 109–116. doi: 10.15430/JCP.2018.23.3.109
  PIM Kinase as an Executional Target in Cancer
  Xinning Zhang, Mengqiu Song, Joydeb Kumar Kundu, Mee-Hyun Lee and Zhen-Zhen Liu

• Bioinformation. 2019; 15(1): 40–45. doi: 10.6026/97320630015040
  A review on PIM kinases in tumors. Housna Arrouchi,1 Wiame Lakhlili,1,* and Azeddine Ibrahimi1 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651028

• Science Advances 18 Sep 2015: Vol. 1, no. 8, e1500221; DOI: 10.1126/sciadv.1500221
  Pim kinases modulate resistance to FLT3 tyrosine kinase inhibitors in FLT3-ITD acute myeloid leukemia
  Alexa S. Green, Thiago T. Maciel, Marie-Anne Hospital, Chae Yin, Fetta Mazed, Elizabeth C. Townsend1, Sylvain Pilorge, Mireille Lambert, Etienne Paubelle, Arnaud Jacquel, Florence Zylbersztejn, Justine Decroocq, Laury Poulain, Pierre Sujobert, Nathalie Jacque, Kevin Adam, Jason C. C. So, Olivier Kosmider , Patrick Auberger, Olivier Hermine, David M. Weinstock1, Catherine Lacombe, Patrick Mayeux, Gary J. Vanasse, Anskar Y. Leung, Ivan C. Moura,†, Didier Bouscary,† and Jerome Tamburini,†,‡

• Leukemia volume 34, pages682–696(2020) FLT3 inhibitors in acute myeloid leukemia: ten frequently asked questions. Ahmad I. Antar, Zaher K. Otrock, Elias Jabbour, Mohamad Mohty & Ali Bazarbachi. https://www.nature.com/articles/s41375-019-0694-3
• Journal of Hematology & Oncology volume 11, Article number: 133 (2018) FLT3 inhibitors in acute myeloid leukemia. Mei Wu, Chuntuan Li & Xiongpeng Zhu.
  https://jhoonline.biomedcentral.com/articles/10.1186/s13045-018-0675-4

SCIENTIFIC PAPERS:

• Oncotarget. 2018 Mar 30; 9(24): 16917–16931. doi: 10.18632/oncotarget.24747
  A novel, dual pan-PIM/FLT3 inhibitor SEL24 exhibits broad therapeutic potential in acute myeloid leukemia
  Wojciech Czardybon, Renata Windak, Aniela Gołas, Michał Gałęzowski, Aleksandra Sabiniarz, Izabela Dolata, Magdalena Salwińska, Paweł Guzik, Magdalena Zawadzka, Ewelina Gabor-Worwa, Bożena Winnik, Małgorzata Żurawska, Ewa Kolasińska, Ewelina Wincza, Marta Bugaj, Monika Danielewicz, Eliza Majewska, Milena Mazan, Grzegorz Dubin, Monika Noyszewska-Kania, Ewa Jabłońska, Maciej Szydłowski, Tomasz Sewastianik, Bartosz Puła, Anna Szumera-Ciećkiewicz, Monika Prochorec-Sobieszek, Elżbieta Mądro, Ewa Lech-Marańda, Krzysztof Warzocha, Jerome Tamburini, Przemysław Juszczyński, and Krzysztof Brzózka

• J Cell Mol Med. 2018 Jul;22(7):3548-3559 Microenvironment-induced PIM kinases promote CXCR4-triggered mTOR pathway required for chronic lymphocytic leukaemia cell migration.
  Białopiotrowicz E, Górniak P, Noyszewska-Kania M, Puła B, Makuch-Łasica H, Nowak G, Bluszcz A, Szydłowski M, Jabłonska E, Piechna K, Sewastianik T, Polak A, Lech-Marańda E, Budziszewska BK, Wasylecka-Juszczyńska M, Borg K, Warzocha K, Czardybon W, Gałęzowski M, Windak R, Brzózka K, Juszczyński P.

• Blood. 2017 Sep 21;130(12):1418-1429 Expression of PIM kinases in Reed-Sternberg cells fosters immune privilege and tumor cell survival in Hodgkin lymphoma
  Szydłowski M, Prochorec-Sobieszek M, Szumera-Ciećkiewicz A, Derezińska E, Hoser G, Wasilewska D, Szymańska-Giemza O, Jabłońska E, Białopiotrowicz E, Sewastianik T, Polak A, Czardybon W, Gałęzowski M, Windak R, Zaucha JM, Warzocha K, Brzózka K, Juszczyński P.